FT-IR MICROSPECTROSCOPY RESEARCH ACTIVITY:
More than 100 presentations (20 at international meetings) have been made by Prof. Wetzel to report experimental results of FT-IR microspectroscopy. Many of these were invited. Several months of first hand experimentation with a borrowed instrument while on a sabbatical in Ottawa, Ontario and with a prototype integrated instrument at the lab of its manufacturer produced sufficient data for a book chapter and a paper in the published proceedings of an international meeting respectively.
Wetzel and KSU chemistry professor Clifton Meloan established the Microbeam Molecular Spectroscopy Laboratory at KSU in 1991 based on the IRΦsTM state-of-the-art infrared integrated microscope/spectrometer. Hatch Funds via the Kansas Agricultural Experiment Station (KAES) on a modest but continuous basis provided graduate research assistantships and expendable supplies. Cooperation with Steven M. LeVine (Associate Professor, University of Kansas Medical Center) began when he brought mouse cerebrum tissue to Manhattan, KS to examine in the laboratory at KSU. Wetzel, LeVine (KUMC), and one professor from the Chemistry, Biology, and Grain Science Departments at Kansas State University received a $300,000 NSF EPSCoR Grant OSR9255223 over three years. This funding resulted in enhancement of capabilities at KSU, increased cooperation between LeVine and Wetzel, and synchrotron experimentation at the National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY within months of the first historic synchrotron infrared microspectroscopy experiment. The grant funded a full time research assistant and GRA=s specifically involved with infrared microbeam work. Besides the presentations, many of the publications listed below (and earlier ones not listed, including two book chapters) were a direct result of the NSF support.
Instrument building experience (mostly in near-IR spectroscopy) includes one patent (AOTF instrument), two active patent applications (polymers), and building an on-line near-IR monitoring system for flour mill QC.
These activities are in evidence by chairmanship of one and participation on several national and international technical committees of the American Association of Cereal Chemists and the International Association for Cereal Chemistry. Other evidence appears in the attached list of journal articles, books and published abstracts of technical presentations at national and international meetings. Recognition in the above areas of specialization is in evidence by appearances as invited speaker at symposia of the Federation of Analytical Chemistry and Spectroscopy Societies (Philadelphia), the International Association for Cereal Chemistry (Vienna, Lausanne, and Porto Carras), the Technicon International Congress (New York), and the NIR Colloquium (New York).
Recognition in chemistry has been within the American Chemical Society (as invited symposium speaker three times); in the Food and Agriculture Division of the ACS (as five-time invited symposium speaker); in the American Society for Testing Materials as an active member of the near-IR task group of the E-13 committee on molecular spectroscopy (two working meetings each year) by the Eastern Analytical Symposium, New York, New York (appeared as symposium invited speaker twice in the past five years on near-IR spectroscopy); by the Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy; in Society of Applied Spectroscopy (represent the Council for Near-Infrared Spectroscopy on the governing board which meets twice a year); in the Federation of Analytical Chemistry and Spectroscopy Societies (invited to arrange and chair an all-day symposium meeting on applied near-infrared spectroscopy
Tandem Mass Spectrometric Determination of Glycolipids in Wheat Endosperm: A New Tool for Breeders to Rank and Select Early Seed Generations. 2014. D.L. Wetzel, Mark D. Boatwright, Allan K. Fritz. Journal of the American Oil Chemists' Society, 91(11):1849-1855.
Positive Assessment of Mill Stream Endosperm Purity Using Chemical Imaging. 2013. D.L. Wetzel. Cereal Foods World 58(3):133-137.
A Quantitative Near Infrared Imaging Study of 1,2,3 Break System Endosperm Yield from Variation of 1BK/2BK Roll Gap Combinations. 2013. Boatwright, M., Gwirtz, J., Posner, E., Wetzel, D. International Miller, 3rd Quarter:35-41.
Mid-IR and Near IR Chemical Imaging: Complementary for Biologic Materials. 2012. Wetzel, David L. Vibrational Spectroscopy, 60: 29-33.
Fourier transform infrared (FT-IR) microspectroscopic genetic expression of the waxy trait in isogenic durum and common wheat. 2012. Brewer, L.; Wetzel, D. Journal of Agriculture and Food Chemistry, 89(2):94-99.
Macromolecular response of individual algal cells to nutrient and altrazine mixtures within biofilms. 2012. Murdock, J.; Wetzel, D. Microb. Ecol., 63(4):761-772.
In situ FT-IR Microspectroscopy and Imaging of Wheat Kernels and Other Grains. 2010. Wetzel, D.; Brewer, L.; Applications of Vibrational Spectroscopy in Food Science, Wiley, London, pp. 367-386.
Confocal Raman and AFM Imaging of Individual Granules of Octenyl Succinate Modified and Natural Waxy Maize Starch. 2010. Wetzel, D.; Shi, Y.C.; Schmidt, U. Vibrational Spectroscopy, 53(1), 173-177.
Granular Solid Formulation Mixing Uniformity via InSb Focal Plane Array Chemical Imaging. 2010. Wetzel, D.; Boatwright, M.; Brewer, L. Vibrational Spectroscopy. 53(1), p. 83-87.
InSb Focal Plane Array Chemical Imaging Enables Assessment of Unit Process Efficiency for Milling Operation. 2010. Wetzel, D.; Posner, E.; Dogan, H. Appl. Spectroscopy, 64(10).
Measuring Cellular Scale Nutrient Distribution in Algal Biofilms with Synchrotron Confocal Infrared Microspectroscopy. 2010. Murdock, J.; Dodds, W.; Reffner, J.; Wetzel, D. Spectroscopy, 25(10), 1-10.
Synchrotron Infrared Confocal Microspectroscopical Detection of Heterogeneity within Chemically Modified Single Starch Granules. 2010. Wetzel, D.; Shi, Y.C.; Reffner, J. Applied Spectroscopy. 64(3), 282-285.
FT-IR microscopic imaging of plant material. 2010. Wetzel, D. Chapter 7 in Infrared and Raman Spectroscopic Imaging, Wiley, pp. 225-258.
Phenotypic expression in wheat revealed using FT-IR microspectroscopy. 2009. Brewer, L. and Wetzel, D. Vibrational Spectroscopy. 52(1): 93-96.
Imminent cardiac risk assessment via optical intravascular biochemical analysis. 2009. Wetzel, D.; Wetzel, L.; Wetzel, M. and Lodder, R. The Analyst. 134(6): 1099-1106.
FT-IR microspectroscopy enhances biological and ecological analysis of algae. 2009. Murdock, J. and Wetzel, D. Applied Spectroscopy Reviews. 44(4): 335-361.
Novel FT-IR microspectroscopic census of simple starch granules for octenyl succinate ester modification. 2009. Bai, Y.; Shi, Y.C. and Wetzel, D. Journal of Agriculture and Food Chemistry. 57(14): 6443-6448.
Innovative FT-IR imaging of protein film secondary structure before and after heat treatment. 2009. Bonwell, E. and Wetzel, D. Journal of Agriculture and Food Chemistry. 57(21): 10067-10072.
Biomedical application of infrared microspectroscopy and imaging by various means. 2008. Wetzel, D. Chapter 3 Journal of Biomedical Vibrational Spectroscopy, Wiley, pp. 39-78.
Reliability of InGaAs focal plane array imaging of wheat germination at early stages. 2008. Koc, H.; Smail, V. and Wetzel, D. Journal of Cereal Science. 48(2): 394-400.
Subcellular localized chemical imaging of benthic algal nutritional content via HgCdTe array FT-IR. 2008. Murdock, J.; Dodds, W. and Wetzel, D. Vibrational Spectroscopy. 48(2): 179-188.
Discrimination of isogenic wheat by InSb focal plane array chemical imaging. 2008. Dogan, H.; Smail, V. and Wetzel, D. Vibrational Spectroscopy. 48(2): 189-195.
Determination of endosperm protein secondary structure in hard wheat breeding lines using synchrotron infrared microspectroscopy. 2008. Bonwell, E.; Fisher, T.; Fritz, A. and Wetzel, D. Vibrational Spectroscopy. 48(2): 76-81.
Imaging! Imaging! Imaging!: Enhancement of Kansas State University Microbeam Molecular Spectroscopy Laboratory. 2007. Wetzel, D. and Dogan, H. NIR News. 18(1): 13
IR microspectroscopic analysis of localized chemical changes in aorta and retina tissue resulting from stimuli. 2007. Wetzel, D. Proceedings Microscopy and Microanalysis. 13(suppl 2): 1382.
Imaging local chemical microstructure of germinated wheat with synchrotron infrared microspectroscopy. 2007. Koc, H. and Wetzel, D. Spectroscopy. 22(10): 24-32
Chemical imaging of intact seeds with NIR focal plane array assists plant breeding. 2006. Smail, V.; Fritz, A. and Wetzel, D. Vibrational Spectroscopy. 42: 215-222.
Preparation of soybean seed samples for FT-IR microspectroscopy. 2005. Miller, S.; Pietrzak, L. and Wetzel, D. Biotechnology and Histochemistry. 870(3-4): 117-121.